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Barang #:
14437041
TBE 10X Liquid Solution, Ultra Pure Grade Solution, 4 Liters/Unit
Barang #:
14437041
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Rincian Produk
| Item Weight | 9 lbs (4.08 kg) |
DESKRIPSI PRODUK
TBE 10X Liquid Solution, Ultra Pure Grade Solution, 4 Liters/Unit
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Tanya & Jawab Pelanggan
-
pertanyaan:
What is TBE 10X Liquid Solution used for?
menjawab: TBE 10X Liquid Solution is primarily used in molecular biology for gel electrophoresis. It serves as a buffering agent that maintains the pH and ionic strength during DNA and RNA separations. The ultra-pure grade ensures minimal contaminants, crucial for sensitive experiments where purity impacts results. For instance, researchers utilize TBE to separate plasmid DNA from restriction enzyme cuts, making it essential for cloning and analysis applications. -
pertanyaan:
How should TBE 10X Liquid Solution be diluted for use?
menjawab: To use TBE 10X Liquid Solution, it must be diluted to a 1X concentration. This is achieved by mixing one part of the TBE 10X solution with nine parts of distilled water. Precise dilution is vital as it directly affects the conductance and effectiveness in experiments like electrophoresis. For example, for a typical 100 mL gel, you would combine 10 mL of TBE 10X with 90 mL of distilled water to ensure optimal performance. -
pertanyaan:
Why is ultra-pure grade important for TBE 10X solutions?
menjawab: Using an ultra-pure grade TBE 10X solution is critical to avoid contamination that can interfere with experimental outcomes. Impurities may lead to unexpected results during gel electrophoresis, skewing data interpretation. For labs focused on precision in DNA or RNA analysis, this purity ensures that assays yield reliable and reproducible results, essential for molecular diagnostics or research development. -
pertanyaan:
Can TBE 10X Liquid Solution be used for RNA electrophoresis?
menjawab: Yes, TBE 10X Liquid Solution is suitable for RNA electrophoresis as it provides the necessary buffering capacity. When preparing RNA samples, the component balances the pH to prevent degradation, ensuring that RNA remains intact during the process. Researchers might use this for assessing RNA integrity in experiments aimed at gene expression studies or cloning applications where accurate RNA quantification is required. -
pertanyaan:
What are the storage requirements for TBE 10X Liquid Solution?
menjawab: TBE 10X Liquid Solution should be stored in a cool, dark place to maintain its stability and prevent degradation over time. Ideally, it should be kept at room temperature, away from direct sunlight and prolonged heat exposure. Proper storage is vital to ensure that the solution remains viable for consistent experimental results in long-term studies or when samples need to be revisited after a period. -
pertanyaan:
Is TBE 10X suitable for low-voltage gel electrophoresis?
menjawab: TBE 10X Liquid Solution is suitable for low-voltage gel electrophoresis, as it maintains conductivity and pH at lower voltages effectively. This condition can yield better resolution and discrimination between DNA fragments of similar sizes. For example, users might prefer low-voltage settings when dealing with high-resolution separations or when visualizing bands for further analysis like cloning efficiency. -
pertanyaan:
What type of gel can be used with TBE 10X solution?
menjawab: TBE 10X Liquid Solution can be used with various gel types, including agarose and polyacrylamide gels. The choice of gel depends on the size of the DNA fragments being separated; agarose is typically used for larger fragments while polyacrylamide is better for smaller ones. Researchers might opt for agarose gels in routine DNA analysis, enabling easy visualization of samples during experiments. -
pertanyaan:
Can I reuse TBE 10X Liquid Solution after electrophoresis?
menjawab: Reusing TBE 10X Liquid Solution after electrophoresis is generally not recommended. The solution may contain contaminants and DNA fragments that could impact the outcome of subsequent experiments. For precise molecular interactions, fresh TBE is essential. However, if assessing multiple gels of similar samples, it might be suitable to reserve for less critical uses, but always investigate contaminant effects. -
pertanyaan:
What safety measures should be taken when using TBE 10X solution?
menjawab: When using TBE 10X Liquid Solution, standard laboratory safety measures should be observed, such as wearing gloves, goggles, and a lab coat. While TBE is generally safe, handling buffers should be done to avoid spills. It’s crucial to ensure no direct contact or ingestion occurs, especially in environments with biological materials. Practicing good lab hygiene helps prevent contamination and ensures safe working conditions. -
pertanyaan:
Where can I buy TBE 10X Liquid Solution, Ultra Pure Grade Solution, 4 Liters in Timor-Leste?
menjawab: You can purchase TBE 10X Liquid Solution, Ultra Pure Grade Solution, 4 Liters from Ubuy in Timor-Leste. Ubuy offers a convenient online platform for sourcing high-quality laboratory solutions and chemicals, ensuring that you get a reliable product for your research needs. They feature a user-friendly site, making it easy to procure essential lab materials with just a few clicks.
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Fitur & Manfaat
- Requires minimal storage space
- Manufactured using ultra pure components
- Rigorous quality control standards
- Convenient concentrations to make 10 liters of 1X solution per pack
- High buffering capacity and ionic strength
- Allows for extended electrophoresis runs without buffer recirculation
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